Operation of the zeiss lsm 510 meta zen version you must sign up to use the microscope or computer every timeno exceptions before attempting to use the microscope. View and download zeiss lsm 510 user manual online. Zeiss lsm 510 meta confocal laser scanning microscope. Dendra2 was expressed under the 35s promoter and spectra were analyzed in the cytoplasm of root hairs by a zeiss lsm510 meta microscope using.
Zeiss lsm 510 meta user manual hege avsnes dale, molecular imaging center, uib. The zeiss lsm 510 confocal system, hardware version 3. Guided tour for lsm 510 and meta welcome to marlin. Lsm tech provides a series of products, upgrades and service contracts for zeiss 510 and 710 confocal laser scanning microscopes. Training guide for zeiss lsm 510 baylor college of medicine. We serve customers located throughout the world, including university, corporate, and government research laboratories. Note that the ssdd mutant protein accumulates in the golgi and that the c181s mutant is absent from the plasma membrane.
Pod shattering resistance associated with domestication is. Lsm 510 confocor 3 single molecule detection the perfect solution for single molecule fluorescence correlation spectroscopy. Antitumor activity of spherical nanoparticles gdyvo 4. Jci insight c3a receptor blockade protects podocytes from. Saturation must be avoided so handling highlow levels of intensity is difficult. Jci insight c3a receptor blockade protects podocytes. Images were taken on live cells grown on labtek chambered coverglass wcvr at 25c. Antilisterial bioactivity and or biofilmformation by.
Ehrlich carcinoma cells were incubated with nanoparticles for 1, 2, 3, and 4 h. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. Sensitive imaging of spectrally overlapping flourochromes. The microscope provides capabilities for confocal analysis of cultured cells, tissue sections and other biological specimens. The lsm 510 and lsm 510 meta laser scanning microscope, including its original accessories and compatible accessories from other manufacturers, may only be used for the purposes and microscopy techniques described in this manual intended use. An integrated imaging and spectroscopic platform for singlemolecule detection article pdf available in current pharmaceutical biotechnology 52.
Lsm 510 upright confocal with meta detector and mp laser isu. Three lasers capable of providing a total of 7 excitation wavelengths 405 uv range dyes, 458, 477, 488, 514, tunable argon for blue dyes, 543 for red dyes, and 633nm for far red dyes. Lsm 510 meta instant spectral imaging undoubtedly the leading light in the lsm 510 range. The zeiss lsm 510 meta laser scanning confocal microscope in biomedical sciences was purchased in 2004 and has been maintained by zeiss. If optical sections are different for each wavelength, go to modechannels and adjust the pinhole so that each channel has the same optical section around 1 airy unit, but one channel will have to be slightly larger. Vba programming for lsm lsm 510 vba programming lsm 510 meta general syntax carl zeiss b 450008 e 1002 63 6 vba programming for lsm 6.
Jun 17, 2002 read sensitive imaging of spectrally overlapping flourochromes using the lsm 510 meta, proceedings of spie on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Zeiss lsm 510 meta inverted confocal microscope m078a smith features include. Nuclear localization of human sod1 and mutant sod1. Zeiss lsm 510 meta laser scanning confocal microscope.
Confocal microscope zeiss lsm 780 system description and. Solveig hehl, tasc carlzeiss jena lsm 510 meta efguided tour april 2002 scanning parameters 2 select frame size as predefined number of pixels or enter own values e. Zinc zn supplementation has been shown to reduce the incidence of diarrhea and to protect animals from intestinal diseases, but the mechanisms of this protective effect against virus infection in vivo have not yet been elucidated. Create a new track with that new track highlighted, click on the recording settings button next to the track portion of the window. A photoreversible proteinpatterning approach for guiding stem cell fate in threedimensional gels. In general, comparison of the diffusion times t 12 from a frap experiment provides qualitative estimates of diffusion rates. Single particle tracking spt is a technique used to locate fluorescent particles with nanometer precision. By means of its emission fingerprinting feature, it can record overlapfree multichannel images even if the emission spectra of the dyes used overlap almost completely see references.
Nuclear localization of human sod1 and mutant sod1specific. A 2 mw, 594 nm hene laser was chosen as the excitation source for the red fp. Microscopy from carl zeiss lsm 5 family colocalization analysis and visualization. Lsm 510 meta, zeiss was used with 458 nm excitation and. Lsm files are the default data export for the zeiss lsm series confocal microscopes e. Zeiss lsm 510 meta guided tour 7 starting the zeiss lsm 510 microscope, software and laser selecting an objective and focusing the microscope selecting an objective and focusing the microscope configuring the laser scanning and detection for confocal image acquisition acquiring a z and time series data storage. Lsm 510 nlo indepth insights especially designed for nonliner optics, i. Zeiss lsm 510 meta zen emory university department of medicine. Confocal fluorescence recovery after photobleaching of. A traditional point scanning confocal laser scanning microscope clsm with excitation from 405 to 633 and multichannel imaging. Imaging field emission scanning electron microscope.
Antilisterial bioactivity and or biofilmformation by compounds from plectranthus ecklonii benth. The lsm 510 meta laser scanning confocal microscope from zeiss is a spectral imaging confocal system. It is hooked to an upright fully motorized axio imager z1 microscope. In addition to the image data, lsm files contain most imaging settings. Guide to confocal microscopy on the zeiss lsm 510 meta nlo. In the orbital tracking method the position of a particle is obtained analyzing the distribution of intensity along a circular orbit scanned around the particle. Jeol jsm7500f laser scanning confocal fluorescence microscope. All images and image stacks were collected using a zeiss lsm 510 meta nlo mounted on an axiovert 200m inverted microscope carl zeiss microimaging, inc. Multiphoton excitation spectra in biological samples. The lsm 5 lets you implement added lsm software functionality and completely automated problem solutions controlled by macros including everything from lsm setting control to image processing and documentation. Open the lsm software in expert mode the software opens like this.
Lsm 510 upright confocal with meta detector and mp laser description the lsm 510 meta laser scanning confocal microscope from zeiss is a spectral imaging confocal system. Fluorescence recovery after photobleaching frap is one of the most widely used approaches to quantitatively estimate diffusion characteristics of molecules in solution and cellular systems. Center on either a zeiss lsm 510 meta nlo equipped with a coherent chameleon multiphoton. Influence of ph on the emission spectra of dendra2. At the end of incubations, living cells were rinsed twice in pbs 1. Highdose dietary zinc oxide mitigates infection with.
Zeiss lsm 510meta confocal laser scanning microscope. Selected references 27 specifications 28 system overview 30. Sections are stained with hoechst33342 and images were captured on a zeiss lsm 510 meta inverted confocal microscope using lsm software. There is loss of the central nucleolus, cajal bodies, and surrounding annulus. It is a stateoftheart confocal microscope that is ideal for multicolor live cell imaging 5d imaging. Start lsm click on multitracking in scan configuration.
The significance of tumor cells incubation with spherical nanoparticles based on orthovanadates of rareearth elements activated by europium gdyvo4. Most of the nuclear space appears to be composed of e with a few densely staining areas of h and ic. Here we introduce a new configuration of the zeiss lsm 510 laser scanning confocal microscope, optimized for live cell imaging of green fluorescent protein gfp together with spectral variants such as mrfp1 and mcherry using standard photo. Page 1 lsm 510 and lsm 510 meta laser scanning microscopes operating manual release 3. Dendra2 was expressed under the 35s promoter and spectra were analyzed in the cytoplasm of root hairs by a zeiss lsm 510 meta microscope using. Zeiss lsm 510 meta zen emory university department of. Largearea blown bubble films of aligned nanowires and. Transmissible gastroenteritis virus tgev causes diarrhea in piglets with an agedependent decrease of severity. Differential requirement for dock2 in migration of. Zeiss lsm510 meta confocal microscope system axioplan 2 stand this system has 458nm, 488nm, 514nm, 543nm and 633nm laser lines. Before looking at the sample in the microscope press the vis button.
Microscopy from carl zeiss lsm 5 family unc school of. Turn on remote control box right side of microscope, and then computer 3. Zeiss lsm510 meta confocal microscope system axioplan 2. This lsm 510 lsm 510 meta lsm 510 nlo quick guide describes the basic operation of the lsm 510 lsm 510 meta lsm 510 nlo laser scanning microscope with the zen 2009 software. Pod shattering resistance was selected for during the domestication of the soybean but the genes involved are unknown. Lsm 510 and lsm 510 meta laser scanning microscopes. A new configuration of the zeiss lsm 510 for simultaneous. Configuration defining spectral range scan control settings optimal pinhole size detector gain, amplitude offset range indicator emission fingerprinting 1.
The purpose of this document is to guide the user to get started with the system as quick as possible in order to obtain some first images from his samples. One stack was captured at 458nm excitation wavelength, which predominantly excites the donor, and a second one was captured at the excitation wavelength of the acceptor 514 nm. Lsm 510 and lsm 510 meta laser scanning microscopes your first choice in cell biology research microscopy from carl zeiss mk4 mk4. Turn on epifluorescence power supply located to the left of microscope. It is an inverted microscope suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and a small stage incubator for co2 supply. Zeiss lsm 510 meta user manual universitetet i bergen. J bm pdcs and mdcs were sorted and analyzed for the.
Meta 3 turn the key on the chameleon to the on position, if needed dapi, 2 photon ex switching on the zeiss lsm 510 confocal microscope. M was added to podocytes plated on type i collagencoated glass coverslips. Microscope confocal lsm510 meta welcome to the zeiss lsm 510 meta confocal tutorial. Horiba jobinyvon nanolog spectrofluorimeter electrochemical workstation. The expression of gc or plasma b cells were estimated by comparing the mean fluorescence intensity using lsm 510 meta, carl zeiss software. Jan 26, 2012 note that the ssdd mutant protein accumulates in the golgi and that the c181s mutant is absent from the plasma membrane. Lsm is zeiss proprietary image format based on tiff.
Zeiss lsm 510 meta 2002 leica sp5 x 2010 zeiss lsm 780 2010 zeiss lsm 7mp 2009. Confocal laser scanning microscopy principles microscopy from carl zeiss optical image formation electronic signal processing authorssources references. The zeiss lsm 510 meta is a confocal pointscanning microscope able to generate highresolution threedimensional images of thick specimens. This opens a dialog box of all your previously aligned single tracks.
Lsm 510 upright confocal with meta detector and mp laser. Largearea blown bubble films of aligned nanowires and carbon nanotubes guihua yu1. Solveig hehl, tasc carlzeiss jena lsm 510 meta efguided tour april 2002 emission fingerprinting guided tour through. The confocal images were acquired on the zeiss lsm 510 nlo meta using the planapochromat 63. Zeiss lsm 510 meta inverted confocal microscope m078a smith. Login using your hawkid, password and domain allows you to save data to your shared drive if available to you. The system provides high resolution images of fluorescent molecules with improved signal noise ratio compared to wide field fluorescence microscopy. Briefly, in the last 30 minutes of the stimuli, mitotracker 250 nm or jc1 5.
A photoreversible protein patterning approach for guiding stem cell fate in threedimensional gels. In 2001, however, the lsm 510 meta solved this problem by means of the awardwinning meta. The lsm 510 the first system of the lsm 5 family and a major breakthrough in confocal imaging and analysis. Read sensitive imaging of spectrally overlapping flourochromes using the lsm 510 meta, proceedings of spie on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Nanophotonics and biofunctional structures capabilities. These can be viewed and extracted using additional tools like the lsm toolbox for fiji. The zeiss lsm510 metafcs is situated in the center for cell signaling, 7 th floor, hospital west. Orbital single particle tracking on a commercial confocal.
View and download zeiss lsm 510 operating manual online. Application of spectral imaging microscopy in cytomics and. Only one detector gain can be used so it is necessary to have signals with. On zeiss lsm 510 meta, select scan controlz stackoptical sliceoptimal interval. Lsm 510 introduction lsm 510 meta carl zeiss b 450008 e 1002 iii how to make best use of the lsm 510 operating instructions a few symbols in these operating instructions will help you to recognize the nature and purpose of. Do the spectrum references of the different fluorochromes and. A new configuration of the zeiss lsm 510 for simultaneous optical separation of green and red fluorescent protein pairs. Inhibiting the palmitoylationdepalmitoylation cycle. Lsm 510 track settings university of california, berkeley. Jasco j815 electron paramagnetic resonance spectroscopy. Zeiss lsm 510 meta guided tour 8 starting the zeiss lsm 510 microscope, software and laser selecting an objective and focusing the microscope selecting an objective and focusing the microscope configuring the laser scanning and detection for confocal image acquisition acquiring a z and time series data storage. Carl zeiss macro description lsm 510 lsm 510 meta 1 may 2005 macro solutions.